Microplate Assay Measurement of Cytochrome P450-Carbon Monoxide Complexes
نویسندگان
چکیده
منابع مشابه
Drug-enhanced carbon monoxide production from heme by cytochrome P450 reductase
Carbon monoxide (CO) formed endogenously is considered to be cytoprotective, and the vast majority of CO formation is attributed to the degradation of heme by heme oxygenases-1 and -2 (HO-1, HO-2). Previously, we observed that brain microsomes containing HO-2 produced many-fold more CO in the presence of menadione and its congeners; herein we explored these observations further. We determined t...
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Recent findings that heme oxygenase-1 can be induced by oxidative stress and inflammation in many different cellular systems, and that carbon monoxide (CO) produced as a by-product of this enzyme is a signaling molecule, have generated a major research area with hundreds of studies published over the last few years. The measurement of expired CO concentration has been used in humans as a biomar...
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Mercaptide anions form exclusively penta-coordinate heme complexes [RS-heme] in polar and nonpolar solution over a wide range of mercaptide concentration. These complexes have a Soret peak at 408 nm and a formation constant of about 2.5 X 10(4) M(-1), and combine with CO to give a CO-cytochrome P-450 type spectrum. Kinetics of CO binding to mercaptide-heme complexes [RS-heme] have been studied ...
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ژورنال
عنوان ژورنال: BMB Reports
سال: 2003
ISSN: 1976-6696
DOI: 10.5483/bmbrep.2003.36.3.332